Ontology highlight
ABSTRACT:
SUBMITTER: Steenhuis M
PROVIDER: S-EPMC7698014 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Steenhuis Maurice M Ten Hagen-Jongman Corinne M CM van Ulsen Peter P Luirink Joen J
Antibiotics (Basel, Switzerland) 20201113 11
The structural integrity of the Gram-negative cell envelope is guarded by several stress responses, such as the σ<sup>E</sup>, Cpx and Rcs systems. Here, we report on assays that monitor these responses in <i>E. coli</i> upon addition of antibacterial compounds. Interestingly, compromised peptidoglycan synthesis, outer membrane biogenesis and LPS integrity predominantly activated the Rcs response, which we developed into a robust HTS (high-throughput screening) assay that is suited for phenotypi ...[more]