Soybean Viromes in the Republic of Korea Revealed by RT-PCR and Next-Generation Sequencing.

ABSTRACT: Soybean (Glycine max L.) is one of the most important crop plants in the Republic of Korea. Here, we conducted a soybean virome study. We harvested a total of 172 soybean leaf samples showing disease symptoms from major soybean-growing regions in the Republic of Korea. Individual samples were examined for virus infection by RT-PCR. Moreover, we generated eight libraries representing eight provinces by pooling samples and four libraries from single samples. RNA-seq followed by bioinformatics analyses revealed 10 different RNA viruses infecting soybean. The proportion of viral reads in each transcriptome ranged from 0.2 to 31.7%. Coinfection of different viruses in soybean plants was very common. There was a single dominant virus in each province, and this geographical difference migh

SUBMITTER: Jo Y 

PROVIDER: S-EPMC7698195 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature