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Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical.


ABSTRACT: Chemically synthesized retinyl palmitate has been widely used in the cosmetic and biotechnology industry. In this study, we aimed to demonstrate the microbial production of retinyl palmitate and the benefits of microbial retinyl palmitate in skin physiology. A heterologous retinyl palmitate biosynthesis pathway was reconstructed in metabolically engineered Escherichia coli using synthetic expression modules from Pantoea agglomerans, Salinibacter ruber, and Homo sapiens. High production of retinyl palmitate (69.96 ± 2.64 mg/L) was obtained using a fed-batch fermentation process. Moreover, application of purified microbial retinyl palmitate to human foreskin HS68 fibroblasts led to increased cellular retinoic acid-binding protein 2 (CRABP2) mRNA level [1.7-fold (p = 0.001) at 100 ?g/mL], acceleration of cell proliferation, and enhancement of procollagen synthesis [111% (p < 0.05) at 100 ?g/mL], strongly indicating an anti-ageing-related effect of this substance. These results would pave the way for large-scale production of retinyl palmitate in microbial systems and represent the first evidence for the application of microbial retinyl palmitate as a cosmeceutical.

SUBMITTER: Choi BH 

PROVIDER: S-EPMC7698207 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical.

Choi Bo Hyun BH   Hwang Hee Jin HJ   Lee Ji Eun JE   Oh Soon Hwan SH   Hwang Jae Sung JS   Lee Bun Yeoul BY   Lee Pyung Cheon PC  

Antioxidants (Basel, Switzerland) 20201114 11


Chemically synthesized retinyl palmitate has been widely used in the cosmetic and biotechnology industry. In this study, we aimed to demonstrate the microbial production of retinyl palmitate and the benefits of microbial retinyl palmitate in skin physiology. A heterologous retinyl palmitate biosynthesis pathway was reconstructed in metabolically engineered <i>Escherichia coli</i> using synthetic expression modules from <i>Pantoea agglomerans</i>, <i>Salinibacter ruber</i>, and <i>Homo sapiens</i  ...[more]

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