Dataset Information

Highly Efficient Antimicrobial Activity of Cu_xFe_yO_z Nanoparticles against Important Human Pathogens.

ABSTRACT: The development of innovative antimicrobial materials is crucial in thwarting infectious diseases caused by microbes, as drug-resistant pathogens are increasing in both number and capacity to detoxify the antimicrobial drugs used today. An ideal antimicrobial material should inhibit a wide variety of bacteria in a short period of time, be less or not toxic to normal cells, and the fabrication or synthesis process should be cheap and easy. We report a one-step microwave-assisted hydrothermal synthesis of mixed composite Cu_xFe_yO_z (Fe₂O₃/Cu₂O/CuO/CuFe₂O) nanoparticles (NPs) as an excellent antimicrobial material. The 1 mg/mL Cu_xFe_yO_z NPs with the composition 36% CuFeO₂, 28% Cu₂O and 36% Fe₂O₃ have a general antimicrobial activity greater than 5 log reduction within 4 h against nine important human pathogenic bacteria (including drug-resistant bacteria as well as Gram-positive and Gram-negative strains). For example, they induced a >9 log reduction in Escherichia coli B viability after 15 min of incubation, and an ~8 log reduction in multidrug-resistant Klebsiella pneumoniae after 4 h incubation. Cytotoxicity tests against mouse fibroblast cells showed about 74% viability when exposed to 1 mg/mL Cu_xFe_yO_z NPs for 24 h, compared to the 20% viability for 1 mg/mL pure Cu₂O NPs synthesized by the same method. These results show that the Cu_xFe_yO_z composite NPs are a highly efficient, low-toxicity and cheap antimicrobial material that has promising potential for applications in medical and food safety.

SUBMITTER: Zhu L

PROVIDER: S-EPMC7699552 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Highly Efficient Antimicrobial Activity of CuxFeyOz Nanoparticles against Important Human Pathogens.

Zhu Lu L Pearson David W DW Benoit Stéphane L SL Xie Jing J Pant Jitendra J Yang Yanjun Y Mondal Arnab A Handa Hitesh H Howe Jane Y JY Hung Yen-Con YC Vidal Jorge E JE Maier Robert J RJ Zhao Yiping Y

Nanomaterials (Basel, Switzerland) 20201120 11

The development of innovative antimicrobial materials is crucial in thwarting infectious diseases caused by microbes, as drug-resistant pathogens are increasing in both number and capacity to detoxify the antimicrobial drugs used today. An ideal antimicrobial material should inhibit a wide variety of bacteria in a short period of time, be less or not toxic to normal cells, and the fabrication or synthesis process should be cheap and easy. We report a one-step microwave-assisted hydrothermal synt ...[more]

PMID: 33233512

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Project description:Vaginal infections continue to be a serious public health issue, and developing new approaches to address antibiotic-resistant pathogens is an urgent task. The dominant vaginal Lactobacillus species and their active metabolites (e.g., bacteriocins) have the potential to defeat pathogens and help individuals recover from disorders. Here, we describe for the first time a novel lanthipeptide, inecin L, a bacteriocin from Lactobacillus iners with posttranslational modifications. The biosynthetic genes of inecin L were actively transcribed in the vaginal environment. Inecin L was active against the prevailing vaginal pathogens, such as Gardnerella vaginalis and Streptococcus agalactiae, at nanomolar concentrations. We demonstrated that the antibacterial activity of inecin L was closely related to the N terminus and the positively charged His13 residue. In addition, inecin L was a bactericidal lanthipeptide that showed little effect on the cytoplasmic membrane but inhibited the cell wall biosynthesis. Thus, the present work characterizes a new antimicrobial lanthipeptide from a predominant species of the human vaginal microbiota. IMPORTANCE The human vaginal microbiota plays essential roles in preventing pathogenic bacteria, fungi, and viruses from invading. The dominant vaginal Lactobacillus species show great potential to be developed as probiotics. However, the molecular mechanisms (such as bioactive molecules and their modes of action) involved in the probiotic properties remain to be determined. Our work describes the first lanthipeptide molecule from the dominant Lactobacillus iners. Additionally, inecin L is the only lanthipeptide found among the vaginal lactobacilli thus far. Inecin L shows strong antimicrobial activity toward the prevalent vaginal pathogens and antibiotic-resistant strains, suggesting that inecin L is a potent antibacterial molecule for drug development. In addition, our results show that inecin L exhibits specific antibacterial activity related to the residues in the N-terminal region and ring A, which will contribute to structure-activity relationship studies in lacticin 481-like lanthipeptides.

Dataset Information

Highly Efficient Antimicrobial Activity of Cu_xFe_yO_z Nanoparticles against Important Human Pathogens.

Publications

Highly Efficient Antimicrobial Activity of Cu<i><sub>x</sub></i>Fe<i><sub>y</sub></i>O<i><sub>z</sub></i> Nanoparticles against Important Human Pathogens.

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