CRISPR RNA-guided autonomous delivery of Cas9.
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ABSTRACT: Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contemporary efforts aimed at developing and deploying this enzyme for programmable genetic editing.
SUBMITTER: Wilkinson RA
PROVIDER: S-EPMC7703833 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
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