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CRISPR RNA-guided autonomous delivery of Cas9.

ABSTRACT: Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contemporary efforts aimed at developing and deploying this enzyme for programmable genetic editing.

SUBMITTER: Wilkinson RA

PROVIDER: S-EPMC7703833 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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CRISPR RNA-guided autonomous delivery of Cas9.

Wilkinson Royce A RA Martin Coleman C Nemudryi Artem A AA Wiedenheft Blake B

Nature structural & molecular biology 20181231 1

Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contem ...[more]

PMID: 30598555

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