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RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

ABSTRACT: Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

SUBMITTER: Perez-Pinera P

PROVIDER: S-EPMC3911785 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

Perez-Pinera Pablo P Kocak D Dewran DD Vockley Christopher M CM Adler Andrew F AF Kabadi Ami M AM Polstein Lauren R LR Thakore Pratiksha I PI Glass Katherine A KA Ousterout David G DG Leong Kam W KW Guilak Farshid F Crawford Gregory E GE Reddy Timothy E TE Gersbach Charles A CA

Nature methods 20130725 10

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided ge ...[more]

PMID: 23892895

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