Ontology highlight
ABSTRACT:
SUBMITTER: Nordeen SA
PROVIDER: S-EPMC7710722 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Nordeen Sarah A SA Andersen Kasper R KR Knockenhauer Kevin E KE Ingram Jessica R JR Ploegh Hidde L HL Schwartz Thomas U TU
Nature communications 20201202 1
Nuclear pore complexes (NPCs) are the main conduits for molecular exchange across the nuclear envelope. The NPC is a modular assembly of ~500 individual proteins, called nucleoporins or nups. Most scaffolding nups are organized in two multimeric subcomplexes, the Nup84 or Y complex and the Nic96 or inner ring complex. Working in S. cerevisiae, and to study the assembly of these two essential subcomplexes, we here develop a set of twelve nanobodies that recognize seven constituent nucleoporins of ...[more]