Browse
Submit Data
Databases
API
Help

Dataset Information

23 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metagenome-Assembled Genome Sequence of Dolichospermum circinale Strain Clear-D4, Assembled from a Harmful Cyanobacterial Bloom Enrichment Culture.

ABSTRACT: Dolichospermum circinale (formerly Anabaena circinale) is a significant harmful algal bloom species. We report the draft metagenome-assembled genome (MAG) for a strain of D. circinale (Clear-D4) obtained from an enrichment culture. The genome sequence comprises 5,029,933?bp in 560 contigs with a GC content of 37%.

SUBMITTER: Florea KM

PROVIDER: S-EPMC7714853 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Metagenome-Assembled Genome Sequence of Dolichospermum circinale Strain Clear-D4, Assembled from a Harmful Cyanobacterial Bloom Enrichment Culture.

Florea Kyra M KM Thrash J Cameron JC Webb Eric A EA

Microbiology resource announcements 20201203 49

Dolichospermum circinale (formerly Anabaena circinale) is a significant harmful algal bloom species. We report the draft metagenome-assembled genome (MAG) for a strain of D. circinale (Clear-D4) obtained from an enrichment culture. The genome sequence comprises 5,029,933 bp in 560 contigs with a GC content of 37%. ...[more]

PMID: 33272998

Similar Datasets

Metagenome-Assembled Genome Sequence of Kapabacteriales Bacterium Strain Clear-D13, Assembled from a Harmful Algal Bloom Enrichment Culture.

Project description:Metagenomic sequencing of a Dolichospermum circinale enrichment culture resulted in the assembly of several cocultured metagenome-assembled genomes (MAGs). One MAG was affiliated with the class Kapabacteriales and included 5,724,991?bp in 127 contigs with a GC content of 48.4%.

| S-EPMC7714850 | biostudies-literature

Metagenome-Assembled Genome Sequence of Aphanizomenon flos-aquae Strain Clear-A1, Assembled from an Enrichment Culture.

Project description:Aphanizomenon flos-aquae is a significant harmful algal bloom-forming cyanobacterial species. Here, we report the draft genome for a strain of A. flos-aquae (Clear-A1) from a harmful algal bloom enrichment culture. This metagenome-assembled genome (MAG) sequence comprises 4,452,466?bp in 60 contigs with a GC content of 37.1%.

| S-EPMC7714854 | biostudies-literature

Metagenome-Assembled Genome Sequences of Raphidiopsis raciborskii and Planktothrix agardhii from a Cyanobacterial Bloom in Kissena Lake, New York, USA.

Project description:Raphidiopsis raciborskii and Planktothrix agardhii are filamentous, potentially toxin-producing cyanobacteria that form nuisance blooms in fresh waters. Here, we report high-quality metagenome-assembled genome sequences of R. raciborskii and P. agardhii collected from a bloom in Kissena Lake, New York.

| S-EPMC7849711 | biostudies-literature

Cyanobacterial bloom

Project description:Cyanobacterial bloom Other

| PRJNA657047 | ENA

Diel Variation in Gene Expression of the CO2-Concentrating Mechanism during a Harmful Cyanobacterial Bloom.

Project description:Dense phytoplankton blooms in eutrophic waters often experience large daily fluctuations in environmental conditions. We investigated how this diel variation affects in situ gene expression of the CO2-concentrating mechanism (CCM) and other selected genes of the harmful cyanobacterium Microcystis aeruginosa. Photosynthetic activity of the cyanobacterial bloom depleted the dissolved CO2 concentration, raised pH to 10, and caused large diel fluctuations in the bicarbonate and O2 concentration. The Microcystis population consisted of three Ci uptake genotypes that differed in the presence of the low-affinity and high-affinity bicarbonate uptake genes bicA and sbtA. Expression of the bicarbonate uptake genes bicA, sbtA, and cmpA (encoding a subunit of the high-affinity bicarbonate uptake system BCT1), the CCM transcriptional regulator gene ccmR and the photoprotection gene flv4 increased at first daylight and was negatively correlated with the bicarbonate concentration. In contrast, genes of the two CO2 uptake systems were constitutively expressed, whereas expression of the RuBisCO chaperone gene rbcX, the carboxysome gene ccmM, and the photoprotection gene isiA was highest at night and down-regulated during daytime. In total, our results show that the harmful cyanobacterium Microcystis is very responsive to the large diel variations in carbon and light availability often encountered in dense cyanobacterial blooms.

| S-EPMC4840274 | biostudies-literature

A method for examining temporal changes in cyanobacterial harmful algal bloom spatial extent using satellite remote sensing.

Project description:Cyanobacterial harmful algal blooms (CyanoHAB) are thought to be increasing globally over the past few decades, but relatively little quantitative information is available about the spatial extent of blooms. Satellite remote sensing provides a potential technology for identifying cyanoHABs in multiple water bodies and across geo-political boundaries. An assessment method was developed using MEdium Resolution Imaging Spectrometer (MERIS) imagery to quantify cyanoHAB surface area extent, transferable to different spatial areas, in Florida, Ohio, and California for the test period of 2008 to 2012. Temporal assessment was used to evaluate changes in satellite resolvable inland waterbodies for each state of interest. To further assess cyanoHAB risk within the states, the World Health Organization's (WHO) recreational guidance level thresholds were used to categorize surface area of cyanoHABs into three risk categories: low, moderate, and high-risk bloom area. Results showed that in Florida, the area of cyanoHABs increased largely due to observed increases in high-risk bloom area. California exhibited a slight decrease in cyanoHAB extent, primarily attributed to decreases in Northern California. In Ohio (excluding Lake Erie), little change in cyanoHAB surface area was observed. This study uses satellite remote sensing to quantify changes in inland cyanoHAB surface area across numerous water bodies within an entire state. The temporal assessment method developed here will be relevant into the future as it is transferable to the Ocean Land Colour Instrument (OLCI) on Sentinel-3A/3B missions.

| S-EPMC6084444 | biostudies-literature

cyanobacterial bloom metatranscriptome

Project description:cyanobacterial bloom metatranscriptome Raw sequence reads

| PRJNA550452 | ENA

Cyanobacterial bloom samples

Project description:Cyanobacterial bloom samples Metagenome

| PRJNA742118 | ENA

Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture.

Project description:We sequenced the metagenome of a microbial community enriched under strictly anaerobic conditions from wastewater treatment plant-derived digester sludge. The metagenomic analysis of the enrichment revealed that Acetobacterium and methanogenic archaea belonged to the dominant prokaryotes, and genes encoding components of the Wood-Ljungdahl pathway were identified.

| S-EPMC7409856 | biostudies-literature

Metatranscriptome profiling of a harmful algal bloom.

Project description:Metagenomic methods provide a powerful means to investigate complex ecological phenomena. Developed originally for study of Bacteria and Archaea, the application of these methods to eukaryotic microorganisms is yet to be fully realized. Most prior environmental molecular studies of eukaryotes have relied heavily on PCR amplification with eukaryote-specific primers. Here we apply high throughput short-read sequencing of poly-A selected RNA to capture the metatranscriptome of an estuarine dinoflagellate bloom. To validate the metatranscriptome assembly process we simulated metatranscriptomic datasets using short-read sequencing data from clonal cultures of four algae of varying phylogenetic distance. We find that the proportion of chimeric transcripts reconstructed from community transcriptome sequencing is low, suggesting that metatranscriptomic sequencing can be used to accurately reconstruct the transcripts expressed by bloom-forming communities of eukaryotes. To further validate the bloom metatransciptome assembly we compared it to a transcriptomic assembly from a cultured, clonal isolate of the dominant bloom-causing alga and found that the two assemblies are highly similar. Eukaryote-wide phylogenetic analyses reveal the taxonomic composition of the bloom community, which is comprised of several dinoflagellates, ciliates, animals, and fungi. The assembled metatranscriptome reveals the functional genomic composition of a metabolically active community. Highlighting the potential power of these methods, we found that relative transcript abundance patterns suggest that the dominant dinoflagellate might be expressing toxin biosynthesis related genes at a higher level in the presence of competitors, predators and prey compared to it growing in monoculture.

| S-EPMC4255328 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data