Dataset Information
The role of menopause in tenofovir diphosphate and emtricitabine triphosphate concentrations in cervical tissue.
Ontology highlight
ABSTRACT:
Design: We used an ex-vivo approach to compare the tissue concentrations of tenofovir (TFV) diphosphate (TFVdp) and emtricitabine (FTC) triphosphate (FTCtp) in cervical tissues from premenopausal (pre-M) and post-M women.
Method: Cervical explants from 16 pre-M and 11 post-M women were incubated in 10-300??g/ml TFV or FTC for 24?h. Explants were then snap frozen in liquid nitrogen and stored until analysis. TFVdp and FTCtp were quantified using tandem liquid chromatography-mass spectrometry.
Results: Active metabolite concentrations of TFVdp were more than nine-fold lower in post-M explants (P?
Conclusion: These findings could have implications in the use of nucleotide-based PrEP strategies targeted to older women. If validated in vivo, lower exposures of active nucleoside/tide metabolites could mean post-M women need higher doses of TFV-based PrEP to achieve protective efficacy.
SUBMITTER: Nicol MR
PROVIDER: S-EPMC7719012 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Publications
The role of menopause in tenofovir diphosphate and emtricitabine triphosphate concentrations in cervical tissue.
AIDS (London, England) 20180101 1
<h4>Objective</h4>Although postmenopausal (post-M) women have behavioral and biological risk factors for HIV infection, the activity of preexposure prophylaxis (PrEP) agents in older adults has not been well studied.<h4>Design</h4>We used an ex-vivo approach to compare the tissue concentrations of tenofovir (TFV) diphosphate (TFVdp) and emtricitabine (FTC) triphosphate (FTCtp) in cervical tissues from premenopausal (pre-M) and post-M women.<h4>Method</h4>Cervical explants from 16 pre-M and 11 po ...[more]