High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution.

ABSTRACT:

Significance

Axially swept light sheet microscopy is used for deconvolution-free, high-resolution 3D imaging, but usually the axial scan mechanism reduces the top imaging speed. Phased arrays (PAs) for axial scanning enable both high resolution and high speed.

Aim

A high-speed PA with an update rate faster than the camera row read time is used to track the rolling shutter at camera-limited rates.

Approach

The point spread function is evaluated to ensure sub-micron isotropic resolution, and the technique is demonstrated on a live Drosophila embryo.

Results

Isotropic resolution is shown down to 720  ±  55  nm in all three spatial dimensions. With an update rate of 2.85  μs, the PA tracks the camera sensor rolling shutter at camera-limited rates. Features in the Drosophila embryo are resolved clearly compared with the equivalent static light sheet case. The random-access nature of the PA enables a camera sensor readout in the same direction for each frame to maintain even temporal sampling in image sequences with no speed loss.

Conclusions

Use of PAs is compatible with axially swept light sheet microscopy and offers significant improvements in speed.

SUBMITTER: Landry J 

PROVIDER: S-EPMC7720907 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature