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MEMS enabled miniaturized light-sheet microscopy with all optical control.

ABSTRACT: We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm³ and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 435 × 232 × 60 µm³ volumes with 0.25 vps (volumes per second) and minimum lateral and axial resolution of 1.0 µm and 3.8 µm respectively. An open-top geometry allows imaging of samples on flat bottomed holders, allowing integration with microfluidic devices, multi-well plates and slide mounted samples, with applications envisaged in biomedical research and pre-clinical settings.

SUBMITTER: Bakas S

PROVIDER: S-EPMC8266809 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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MEMS enabled miniaturized light-sheet microscopy with all optical control.

Bakas Spyridon S Uttamchandani Deepak D Toshiyoshi Hiroshi H Bauer Ralf R

Scientific reports 20210708 1

We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm<sup>3</sup> and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 435 × 232 × 60 µm<sup>3</sup> volumes ...[more]

PMID: 34238945

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