Dataset Information

¹Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).

ABSTRACT:

Background

Cadmium (Cd) accumulation in crops affects the yield and quality of crops and harms human health. The application of selenium (Se) can reduce the absorption and transport of Cd in winter wheat.

Results

The results showed that increasing Se supply significantly decreased Cd concentration and accumulation in the shoot and root of winter wheat and the root-to-shoot translocation of Cd. Se application increased the root length, surface area and root volume but decreased the average root diameter. Increasing Se supply significantly decreased Cd concentration in the cell wall, soluble fraction and cell organelles in root and shoot. An increase in Se supply inhibited Cd distribution in the organelles of shoot and root but enhanced Cd distribution in the soluble fraction of shoot and the cell wall of root. The Se supply also decreased the proportion of active Cd (ethanol-extractable (FE) Cd and deionized water-extractable (FW) Cd) in root. In addition, the expression of TaNramp5-a, TaNramp5-b, TaHMA3-a, TaHMA3-b and TaHMA2 significantly increased with increasing Cd concentration in root, and the expression of TaNramp5-a, TaNramp5-b and TaHMA2 in root was downregulated by increasing Se supply, regardless of Se supply or Cd stress. The expression of TaHMA3-b in root was significantly downregulated by 10??M Se at both the 5??M and 25??M Cd level but upregulated by 5??M Se at the 25??M Cd level. The expression of TaNramp5-a, TaNramp5-b, TaHMA3-a, TaHMA3-b and TaHMA2 in shoot was downregulated by increasing Se supply at 5??M Cd level, and 5??M Se upregulated the expression of those genes in shoot at 25??M Cd level.

Conclusions

The results confirm that Se application limits Cd accumulation in wheat by regulating the subcellular distribution and chemical forms of Cd in winter wheat tissues, as well as the expression of TaNramp5-a, TaNramp5-b and TaHMA2 in root.

SUBMITTER: Zhu J

PROVIDER: S-EPMC7722431 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

<sup>1</sup>Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).

Zhu Jiaojiao J Zhao Peng P Nie Zhaojun Z Shi Huazhong H Li Chang C Wang Yi Y Qin Shiyu S Qin Xiaoming X Liu Hongen H

BMC plant biology 20201207 1

<h4>Background</h4>Cadmium (Cd) accumulation in crops affects the yield and quality of crops and harms human health. The application of selenium (Se) can reduce the absorption and transport of Cd in winter wheat.<h4>Results</h4>The results showed that increasing Se supply significantly decreased Cd concentration and accumulation in the shoot and root of winter wheat and the root-to-shoot translocation of Cd. Se application increased the root length, surface area and root volume but decreased the ...[more]

PMID: 33287728

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Project description:Androgenesis is potentially the most effective technique for doubled haploid production of wheat. It is not however widely used in breeding programmes due to its main limitation: the genotype dependence. Due to genetic differences between spring and winter wheat, it was assumed that both phenotypes are different in their capacity to conduct androgenesis. And so, the aim of this investigation was to verify the effectiveness of androgenesis induction and plant regeneration of spring and winter wheat genotypes while considering varying amounts of growth hormones in the induction medium. Fifteen genotypes of spring wheat and fifteen of winter wheat were used in the experiment. Six hundred anthers of each of the 30 genotypes were plated and analysed. Previous studies have allowed selection of the best medium for wheat androgenesis and a combination of growth hormones that are the most effective in stimulating microspore proliferation. Therefore, C17 induction media with two combinations of growth hormones were used: I-supplemented only by auxins (2,4-D and dicamba), and II-supplemented by auxin and cytokinin (2,4-D and kinetin). Data was recorded according to the efficiency of androgenic structure formation (ASF), green plant regeneration (GPR), and albino plant regeneration (APR). The results showed that the induction and regeneration of androgenesis in the spring wheat were more efficient than in the winter ones. The spring genotypes formed more androgenic structures and green plants on anthers plated on the medium supplemented only by auxins, in contrast to the winter genotypes which were better induced and regenerated on the medium supplemented by auxin and cytokinin. The study showed that to increase the efficiency of androgenesis, it is necessary to select appropriate factors such as concentration and type of hormones in medium composition, affecting the course of the culturing procedure according to the winter or spring phenotype of donor plants.

Dataset Information

¹Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).

Background

Results

Conclusions

Publications

<sup>1</sup>Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

1Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).

Background

Results

Conclusions

Publications

<sup>1</sup>Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

¹Selenium supply alters the subcellular distribution and chemical forms of cadmium and the expression of transporter genes involved in cadmium uptake and translocation in winter wheat (Triticum aestivum).