Ontology highlight
ABSTRACT:
SUBMITTER: Fukushima S
PROVIDER: S-EPMC7729581 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Fukushima Sachiyo S Farea Manal M Maeta Kazuhiro K Rani Abdul Qawee Mahyoob AQM Fujioka Kazumichi K Nishio Hisahide H Matsuo Masafumi M
International journal of molecular sciences 20201130 23
Splicing reporter minigenes are used in cell-based in vitro splicing studies. Exon skippable antisense oligonucleotide (ASO) has been identified using minigene splicing assays, but these assays include a time- and cost-consuming step of reverse transcription PCR amplification. To make in vitro splicing assay easier, a ready-made minigene (FMv2) amenable to quantitative splicing analysis by fluorescence microscopy was constructed. FMv2 was designed to encode two fluorescence proteins namely, mChe ...[more]