Estrogen-related receptor alpha directly binds to p53 and cooperatively controls colon cancer growth through the regulation of mitochondrial biogenesis and function.

ABSTRACT: Background: Of the genes that control mitochondrial biogenesis and function, ERR? emerges as a druggable metabolic target to be exploited for cancer therapy. Of the genes mutated in cancer, TP53 remains the most elusive to target. A clear understanding of how mitochondrial druggable targets can be accessed to exploit the underlying mechanism(s) explaining how p53-deficient tumors promote cell survival remains elusive.

Methods: We performed protein-protein interaction studies to demonstrate that ERR? binds to p53. Moreover, we used gene silencing and pharmacological approaches in tandem with quantitative proteomics analysis by SWATH-MS to investigate the role of the ERR?/p53 complex in mitochondrial biogenesis and function in colon cancer. Finally, we designed in vitro and in vivo studies to investigate the possibility of targeting colon cancers that exhibit defects in p53.

Results: Here, we are the first to identify a direct protein-protein interaction between the ligand-binding domain (LBD) of ERR? and the C-terminal domain (CTD) of p53. ERR? binds to p53 regardless of p53 mutational status. Furthermore, we show that the ERR? and p53 complex cooperatively control mitochondrial biogenesis and function. Targeting ERR? creates mitochondrial metabolic stresses, such as production of reactive oxygen species (ROS) and mitochondrial membrane permeabilization (MMP), leading to a greater cytotoxic effect that is dependent on the presence of p53. Pharmacological inhibition of ERR? impairs the growth of p53-deficient cells and of p53 mutant patient-derived colon xenografts (PDX).

Conclusions: Therefore, our data suggest that by using the status of the p53 protein as a selection criterion, the ERR?/p53 transcriptional axis can be exploited as a metabolic vulnerability.

SUBMITTER: De Vitto H 

PROVIDER: S-EPMC7731476 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature