Ontology highlight
ABSTRACT:
SUBMITTER: Er-Lukowiak M
PROVIDER: S-EPMC7733020 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Er-Lukowiak Marco M Duan Yinghui Y Rassendren Francois F Ulmann Lauriane L Nicke Annette A Ufer Friederike F Friese Manuel A MA Koch-Nolte Friedrich F Magnus Tim T Rissiek Björn B
iScience 20201127 12
Among laboratory mouse strains many genes are differentially expressed in the same cell population. As consequence, gene targeting in 129-derived embryonic stem cells (ESCs) and backcrossing the modified mice onto the C57BL/6 background can introduce passenger mutations in the close proximity of the targeted gene. Here, we demonstrate that several transgenic mice carry a <i>P2rx7</i> passenger mutation that affects the function of T cells. By the example of P2rx4<sup>tm1Rass</sup> we demonstrate ...[more]