Unknown

Dataset Information

0

Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System.


ABSTRACT: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) RNA-guided endonucleases are powerful new tools for targeted genome engineering. These nucleases provide an efficient and precise method for manipulating eukaryotic genomes; however, delivery of these reagents to specific cell-types remains challenging. Virus-like particles (VLPs) derived from bacteriophage P22, are robust supramolecular protein cage structures with demonstrated utility for cell type-specific delivery of encapsulated cargos. Here, we genetically fuse Cas9 to a truncated form of the P22 scaffold protein, which acts as a template for capsid assembly as well as a specific encapsulation signal for Cas9. Our results indicate that Cas9 and a single-guide RNA are packaged inside the P22 VLP, and activity assays indicate that this RNA-guided endonuclease is functional for sequence-specific cleavage of dsDNA targets. This work demonstrates the potential for developing P22 as a delivery vehicle for cell specific targeting of Cas9.

SUBMITTER: Qazi S 

PROVIDER: S-EPMC7734702 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System.

Qazi Shefah S   Miettinen Heini M HM   Wilkinson Royce A RA   McCoy Kimberly K   Douglas Trevor T   Wiedenheft Blake B  

Molecular pharmaceutics 20160222 3


Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) RNA-guided endonucleases are powerful new tools for targeted genome engineering. These nucleases provide an efficient and precise method for manipulating eukaryotic genomes; however, delivery of these reagents to specific cell-types remains challenging. Virus-like particles (VLPs) derived from bacteriophage P22, are robust supramolecular protein cage structures with demonstrated utility for cell type-specific delivery of encapsul  ...[more]

Similar Datasets

| S-EPMC4499798 | biostudies-literature
| S-EPMC3190671 | biostudies-literature
| S-EPMC5848079 | biostudies-literature
| S-EPMC9618141 | biostudies-literature
| S-EPMC6424964 | biostudies-literature
| S-EPMC6900514 | biostudies-literature
| S-EPMC4820368 | biostudies-literature
| S-EPMC5924900 | biostudies-literature
| S-EPMC3985930 | biostudies-literature
| S-EPMC5238535 | biostudies-other