Ontology highlight
ABSTRACT:
SUBMITTER: Luchinat E
PROVIDER: S-EPMC7735671 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Luchinat Enrico E Barbieri Letizia L Cremonini Matteo M Nocentini Alessio A Supuran Claudiu T CT Banci Lucia L
ACS chemical biology 20200930 10
Candidate drugs rationally designed <i>in vitro</i> often fail due to low efficacy <i>in vivo</i> caused by low tissue availability or because of unwanted side effects. To overcome the limitations of <i>in vitro</i> rational drug design, the binding of candidate drugs to their target needs to be evaluated in the cellular context. Here, we applied in-cell NMR to investigate the binding of a set of approved drugs to the isoform II of carbonic anhydrase (CA) in living human cells. Some compounds we ...[more]