Dataset Information
Chemokines during anaphylaxis: the importance of CCL2 and CCL2-dependent chemotactic activity for basophils.
Ontology highlight
ABSTRACT: Background
The role of chemokines in anaphylaxis is unclear.Methods
We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the anaphylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells.Results
Only CCL2 chemokine levels were significantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P?ConclusionsOur findings imply an important and specific role for CCL2-mediated chemotactic activity in the pathophysiology of human anaphylaxis.
SUBMITTER: Vantur R
PROVIDER: S-EPMC7737350 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Chemokines during anaphylaxis: the importance of CCL2 and CCL2-dependent chemotactic activity for basophils.
Clinical and translational allergy 20201215 1
<h4>Background</h4>The role of chemokines in anaphylaxis is unclear.<h4>Methods</h4>We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time p ...[more]
