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ABSTRACT:
SUBMITTER: Altmannova V
PROVIDER: S-EPMC7737779 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Altmannova Veronika V Blaha Andreas A Astrinidis Susanne S Reichle Heidi H Weir John R JR
Protein science : a publication of the Protein Society 20201013 1
The successful production of recombinant protein for biochemical, biophysical, and structural biological studies critically depends on the correct expression organism. Currently, the most commonly used expression organisms for structural studies are Escherichia coli (~70% of all PDB structures) and the baculovirus/ insect cell expression system (~5% of all PDB structures). While insect cell expression is frequently successful for large eukaryotic proteins, it is relatively expensive and time-con ...[more]