Unknown

Dataset Information

0

β-Lactamase triggered visual detection of bacteria using cephalosporin functionalized biomaterials.


ABSTRACT: We report the conjugation of a chromogenic cephalosporin β-lactamase (βL) substrate to polymers and integration into biomaterials for facile, visual βL detection. Identification of these bacterial enzymes, which are a leading cause of antibiotic resistance, is critical in the treatment of infectious diseases. The βL substrate polymer conjugate undergoes a clear to deep yellow color change upon incubation with common pathogenic Gram-positive and Gram-negative bacteria species. We have demonstrated the feasibility of formulating hydrogels with the βL substrate covalently tethered to a poly(ethylene glycol) (PEG) polymer matrix, exhibiting a visible color change in the presence of βLs. This approach has the potential to be used in diagnostic biomaterials for point-of-care detection of βL-producing bacteria, helping combat the spread of drug resistant microbes.

SUBMITTER: Alkekhia D 

PROVIDER: S-EPMC7739975 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7666973 | biostudies-literature
| S-EPMC7506896 | biostudies-literature
| S-EPMC4803327 | biostudies-literature
| S-EPMC7266096 | biostudies-literature
| S-EPMC9645384 | biostudies-literature
| PRJEB44132 | ENA
| S-EPMC1489774 | biostudies-literature
| S-EPMC5380883 | biostudies-literature
| S-EPMC9723239 | biostudies-literature
| S-EPMC6207675 | biostudies-literature