Dataset Information

Effect of Bitis gabonica and Dendroaspis angusticeps snake venoms on apoptosis-related genes in human thymic epithelial cells.

ABSTRACT:

Background

Certain environmental toxins permanently damage the thymic epithelium, accelerate immune senescence and trigger secondary immune pathologies. However, the exact underlying cellular mechanisms and pathways of permanent immune intoxication remain unknown. The aim of the present study was to demonstrate gene expressional changes of apoptosis-related cellular pathways in human thymic epithelial cells following exposure to snake venom from Bitis gabonica and Dendroaspis angusticeps.

Methods

Snake venoms were characterized by analytical methods including reversed phase high-performance liquid chromatography and sodium dodecyl sulphate-polyacrylamide gel electrophoresis, then applied on human thymic epithelial cells (1889c) for 24 h at 10 ?g/mL (as used in previous TaqMan Array study). Gene expressional changes restricted to apoptosis were assayed by TaqMan Array (Human Apoptosis Plate).

Results

The most prominent gene expressional changes were shown by CASP5 (? 2.5 million-fold, confirmed by dedicated quantitative polymerase chain reaction) and CARD9 (0.016-fold) for B. gabonica, and BIRC7 (6.46-fold) and CASP1 (0.30-fold) for D. angusticeps.

Conclusion

The observed apoptotic environment suggests that pyroptosis may be the dominant pathway through which B. gabonica and D. angusticeps snake venoms trigger thymic epithelial apoptosis following envenomation.

SUBMITTER: Boda F

PROVIDER: S-EPMC7745260 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

Effect of <i>Bitis gabonica</i> and <i>Dendroaspis angusticeps</i> snake venoms on apoptosis-related genes in human thymic epithelial cells.

Boda Francisc F Banfai Krisztina K Garai Kitti K Kovacs Bela B Almasi Attila A Scheffer Dalma D Sinkler Reka Lambertne RL Csonka Robert R Czompoly Tamas T Kvell Krisztian K

The journal of venomous animals and toxins including tropical diseases 20201214

<h4>Background</h4>Certain environmental toxins permanently damage the thymic epithelium, accelerate immune senescence and trigger secondary immune pathologies. However, the exact underlying cellular mechanisms and pathways of permanent immune intoxication remain unknown. The aim of the present study was to demonstrate gene expressional changes of apoptosis-related cellular pathways in human thymic epithelial cells following exposure to snake venom from <i>Bitis gabonica</i> and <i>Dendroaspis a ...[more]

PMID: 33402885

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