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Actin chromobody imaging reveals sub-organellar actin dynamics.

ABSTRACT: The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.

SUBMITTER: Schiavon CR

PROVIDER: S-EPMC7746311 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Actin chromobody imaging reveals sub-organellar actin dynamics.

Schiavon Cara R CR Zhang Tong T Zhao Bing B Moore Andrew S AS Wales Pauline P Andrade Leonardo R LR Wu Melissa M Sung Tsung-Chang TC Dayn Yelena Y Feng Jasmine W JW Quintero Omar A OA Shadel Gerald S GS Grosse Robert R Manor Uri U

Nature methods 20200810 9

The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to ena ...[more]

PMID: 32778832

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