Adaptive optics improves multiphoton super-resolution imaging.
Ontology highlight
ABSTRACT:
SUBMITTER: Zheng W
PROVIDER: S-EPMC7749710 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Publications
Adaptive optics improves multiphoton super-resolution imaging.
Zheng Wei W Wu Yicong Y Winter Peter P Fischer Robert R Nogare Damian Dalle DD Hong Amy A McCormick Chad C Christensen Ryan R Dempsey William P WP Arnold Don B DB Zimmerberg Joshua J Chitnis Ajay A Sellers James J Waterman Clare C Shroff Hari H
Nature methods 20170619 9
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface. ...[more]
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