Dataset Information

T cell phenotypes associated with insulin resistance: results from the Berlin Aging Study II.

ABSTRACT:

Background

Obesity is associated with chronic low-grade inflammation leading to metabolic and cardiovascular diseases, but a subset of obese individuals is considered insulin sensitive (IS). The underlying pathophysiologic mechanisms remain elusive and clinical studies on the relationship between inflammatory markers and metabolically healthy obesity (MHO) are scarce.

Methods

In this cross-sectional analysis, we included a sample of 437 older participants (60-84?years) from the Berlin Aging Study II (BASE-II). Peripheral blood mononuclear cells were isolated, immune cell subsets were analyzed with multiparameter flow cytometry and systemic cytokine levels were measured. Immune cell parameters were correlated with metabolic measures and multiple linear regression analysis was conducted and adjusted for various demographic and clinical factors.

Results

We found that frequencies of naïve and memory CD4⁺ and CD8⁺ T cells inversely correlated with measures for insulin sensitivity in the older population. Moreover, the percentages of naïve CD4⁺ and CD8⁺ T cells were significantly higher, whereas activated T cells and IL-6 levels were lower in IS compared to insulin resistant (IR) obese individuals. The percentages of naïve CD4⁺ and CD8⁺ T cells were predictive for impaired insulin sensitivity (ß?=?0.16, p?=?0.01 and ß?=?0.11, p?=?0.04), and the association of naïve CD4⁺ T cells with insulin sensitivity persisted after multivariate adjustment (ß?=?0.14, p?=?0.02).

Conclusions

These findings support the hypothesis that parameters of systemic inflammation can differentiate IS from IR obese individuals that are at higher risk for cardiometabolic diseases and may have clinical implications with regard to obesity treatment stratification.

Trial registration

DRKS00009277 . Registered 31 August 2015 - Retrospectively registered.

SUBMITTER: Sbierski-Kind J

PROVIDER: S-EPMC7751110 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

T cell phenotypes associated with insulin resistance: results from the Berlin Aging Study II.

Sbierski-Kind Julia J Goldeck David D Buchmann Nikolaus N Spranger Joachim J Volk Hans-Dieter HD Steinhagen-Thiessen Elisabeth E Pawelec Graham G Demuth Ilja I Spira Dominik D

Immunity & ageing : I & A 20201221 1

<h4>Background</h4>Obesity is associated with chronic low-grade inflammation leading to metabolic and cardiovascular diseases, but a subset of obese individuals is considered insulin sensitive (IS). The underlying pathophysiologic mechanisms remain elusive and clinical studies on the relationship between inflammatory markers and metabolically healthy obesity (MHO) are scarce.<h4>Methods</h4>In this cross-sectional analysis, we included a sample of 437 older participants (60-84 years) from the Be ...[more]

PMID: 33349270

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Project description:BACKGROUND:Walking ability is an important prerequisite for activity, social participation and independent living. While in most healthy adults, this ability can be assumed as given, limitations in walking ability occur with increasing age. Furthermore, slow walking speed is linked to several chronic conditions and overall morbidity. Measurements of gait parameters can be used as a proxy to detect functional decline and onset of chronic conditions. Up to now, gait characteristics used for this purpose are measured in standardized laboratory settings. There is some evidence, however, that long-term measurements of gait parameters in the living environment have some advantages over short-term laboratory measurements. METHODS:We evaluated cross-sectional data from an accelerometric sensor worn in a subgroup of 554 participants of the Berlin Aging Study II (BASE-II). Data from the two BASE-II age groups (age between 22-36 years and 60-79 years) were used for the current analysis of accelerometric data for a minimum of two days and a maximum of ten days were available. Real world walking speed, number of steps, maximum coherent distance and total distance were derived as average data per day. Linear regression analyses were performed on the different gait parameters in order to identify significant determinants. Additionally, Mann-Whitney-U-tests were performed to detect sex-specific differences. RESULTS:Age showed to be significantly associated with real world walking speed and with the total distance covered per day, while BMI contributed negatively to the number of walking steps, maximum coherent distance and total distance walked. Additionally, sex was associated with walking steps. However, R2-values for all models were low. Overall, women had significantly more walking steps and a larger coherent distance per day when compared to men. When separated by age group, this difference was significant only in the older participants. Additionally, walking speed was significantly higher in women compared to men in the subgroup of older people. CONCLUSIONS:Age- and sex-specific differences have to be considered when objective gait parameters are measured, e.g. in the context of clinical risk assessment. For this purpose normative data, differentiating for age and sex would have to be established to allow reliable classification of long-term measurements of gait.

Dataset Information

T cell phenotypes associated with insulin resistance: results from the Berlin Aging Study II.

Background

Methods

Results

Conclusions

Trial registration

Publications

T cell phenotypes associated with insulin resistance: results from the Berlin Aging Study II.

Similar Datasets

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