Ontology highlight
ABSTRACT:
SUBMITTER: Pulupa J
PROVIDER: S-EPMC7752133 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Pulupa Joan J Prior Harriet H Johnson Daniel S DS Simon Sanford M SM
eLife 20201221
While the static structure of the nuclear pore complex (NPC) continues to be refined with cryo-EM and x-ray crystallography, <i>in vivo</i> conformational changes of the NPC remain under-explored. We developed sensors that report on the orientation of NPC components by rigidly conjugating mEGFP to different NPC proteins. Our studies show conformational changes to select domains of nucleoporins (Nups) within the inner ring (Nup54, Nup58, Nup62) when transport through the NPC is perturbed and no c ...[more]