Dataset Information

The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.

ABSTRACT: VERNALIZATION2 (VRN2), an angiosperm-specific subunit of the polycomb repressive complex 2 (PRC2), is an oxygen (O₂ )-regulated target of the PCO branch of the PRT6 N-degron pathway of ubiquitin-mediated proteolysis. How this post-translational regulation coordinates VRN2 activity remains to be fully established. Here we use Arabidopsis thaliana ecotypes, mutants and transgenic lines to determine how control of VRN2 stability contributes to its functions during plant development. VRN2 localizes to endogenous hypoxic regions in aerial and root tissues. In the shoot apex, VRN2 differentially modulates flowering time dependent on photoperiod, whilst its presence in lateral root primordia and the root apical meristem negatively regulates root system architecture. Ectopic accumulation of VRN2 does not enhance its effects on flowering, but does potentiate its repressive effects on root growth. In late-flowering vernalization-dependent ecotypes, VRN2 is only active outside meristems when its proteolysis is inhibited in response to cold exposure, as its function requires concomitant cold-triggered increases in other PRC2 subunits and cofactors. We conclude that the O₂ -sensitive N-degron of VRN2 has a dual function, confining VRN2 to meristems and primordia, where it has specific developmental roles, whilst also permitting broad accumulation outside of meristems in response to environmental cues, leading to other functions.

SUBMITTER: Labandera AM

PROVIDER: S-EPMC7754370 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.

Labandera Anne-Marie AM Tedds Hannah M HM Bailey Mark M Sprigg Colleen C Etherington Ross D RD Akintewe Olunwatunmise O Kalleechurn Geetika G Holdsworth Michael J MJ Gibbs Daniel J DJ

The New phytologist 20200316 1

VERNALIZATION2 (VRN2), an angiosperm-specific subunit of the polycomb repressive complex 2 (PRC2), is an oxygen (O<sub>2</sub> )-regulated target of the PCO branch of the PRT6 N-degron pathway of ubiquitin-mediated proteolysis. How this post-translational regulation coordinates VRN2 activity remains to be fully established. Here we use Arabidopsis thaliana ecotypes, mutants and transgenic lines to determine how control of VRN2 stability contributes to its functions during plant development. VRN2 ...[more]

PMID: 32043277

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Project description:Previous studies have indicated that a niche variation scheme, similar to the periodic table of elements, can be constructed based on functional traits. The periodic table of niches for species is defined as a multidimensional ordination scheme of niche relationships and their orders in a specific biotic community. Comparing functional trait-based niches is extremely useful in theoretical studies of plant ecological strategies, community assembly, and the geographic differentiation of biomes across different climatic zones. Here, data for 11 functional traits belonging to three fundamental niche dimensions (leaf economy, mechanical support, and reproductive phenology) were compiled for 215 woody species from forests across four climatic zones (tropical, subtropical, warm-temperate, and cold-temperate). We constructed the periodic table of niches based on the functional traits of plants in different communities and explored their variations among biomes. A principal component analysis (PCA) was performed to derive the dominant gradients of trait combinations for each individual niche dimensional dataset. Then species scores for the first two axes (PC1 and PC2) were used as inputs for a second PCA to ordinate species in the continuous niche space constrained by the three niche dimensions. Changes in the functional niches of plants from the four biomes along the PC1 and PC2 of niche space were examined based on species scores. Leaf economy was the dominant functional dimension in the plant niche space, followed by mechanical support. Considerable niche convergences among different species were found in the niche space for each biome, except cold-temperate forest. The species niches varied mainly with the increasing specific leaf area/decreasing stem tissue density along PC1, and with the decrease of leaf area/plant size along PC2 from tropical to temperate forests, suggesting that the ecological strategies of plants in the four biomes changed from conservative to acquisitive with an increase in latitude. Our results confirmed that the periodic table of niches does exist and can be constructed by major functional dimensions composed of dominant functional traits. The periodic table of niches effectively reflects the changes of ecological strategies of plant species in biomes across different climatic zones.

Dataset Information

The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.

Publications

The PRT6 N-degron pathway restricts VERNALIZATION 2 to endogenous hypoxic niches to modulate plant development.

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