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Dark accelerates dissolved inorganic phosphorus release of high-density cyanobacteria.

ABSTRACT: Bloom-forming cyanobacteria dramatically influence nutrient cycling in eutrophic freshwater lakes. The phosphorus (P) assimilation and release of bloom-forming cyanobacteria significantly may also affect the phosphorus source and amounts in water. To understand the phosphorus release process of bloom-forming cyanobacteria below the accumulated surface and sedimentary bloom-forming cyanobacteria, the degradation of bloom-forming cyanobacteria dominated by Microcystis spp. at different cell density in the dark was investigated over a 25-day microcosm experiment. The dissolved inorganic phosphorus (DIP) and dissolved total phosphorus (DTP) contents increased with the increment of cyanobacterial density, and the dark status markedly increased the proportion of DIP in water during the decline period of bloom-forming cyanobacteria. Meanwhile, the process of cyanobacterial apoptosis accompanied by the changes of malondialdehyde (MDA) and phosphatase (AKP) contents, and the increases of superoxide dismutase (SOD) and catalase (CAT) activities of cyanobacteria in the dark, especially in low-density groups (5.23×108 cells L-1), which further affect the physicochemical water parameters. Moreover, the DIP release from high-density cyanobacteria (7.86×107 cells L-1~5.23×108 cells L-1) resulted from the relative abundance of organophosphorus degrading bacteria in the dark. Therefore, the fast decay of cyanobacteria in the dark could accelerate DIP release, the high DIP release amount from accumulated bloom-cyanobacteria provide adequate P quickly for the sustained growth of cyanobacteria.

SUBMITTER: Wang M

PROVIDER: S-EPMC7755282 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Dark accelerates dissolved inorganic phosphorus release of high-density cyanobacteria.

Wang Mengmeng M Zhang Huifen H Chen Menggaoshan M Yang Liuyan L Yang Yichen Y

PloS one 20201222 12

Bloom-forming cyanobacteria dramatically influence nutrient cycling in eutrophic freshwater lakes. The phosphorus (P) assimilation and release of bloom-forming cyanobacteria significantly may also affect the phosphorus source and amounts in water. To understand the phosphorus release process of bloom-forming cyanobacteria below the accumulated surface and sedimentary bloom-forming cyanobacteria, the degradation of bloom-forming cyanobacteria dominated by Microcystis spp. at different cell densit ...[more]

PMID: 33351804

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Project description:The hydrothermal vent gammaproteobacterium Thiomicrospira crunogena inhabits an unstable environment and must endure dramatic sweeps in habitat chemistry. This sulfur chemolithoautotroph responds to changes in dissolved inorganic carbon (DIC; = CO2 + HCO3- + CO3-2) availability with a carbon concentrating mechanism (CCM), in which whole-cell affinity for DIC, as well as the intracellular DIC concentration, increase substantially under DIC-limitation. To determine whether this CCM is regulated at the level of transcription, cells cultivated under high-DIC conditions in chemostats were resuspended in growth medium with low concentrations of DIC, and CCM development was tracked in the presence and absence of RNA polymerase inhibitor rifampicin. The induction of the CCM, as measured by silicone oil centrifugation, was hindered in the presence of rifampicin. Similar results were observed for carboxysome gene transcription and assembly, as assayed by qRT-PCR and transmission electron microscopy, respectively. Genome-wide transcription patterns, assayed via microarrays, were compared for cells grown under DIC-limitation versus ammonia limitation. In addition to carboxysome genes, two novel genes (Tcr_1019 and Tcr_1315), present in other organisms including chemolithoautotrophs, but whose function(s) have not been elucidated in any organism, were found to be upregulated under low-DIC conditions. Likewise, under ammonia limitation, in addition to the expected enhancement of ammonia transporter and PII-gene transcription, the transcription of two novel genes was measurably enhanced (Tcr_0466 and Tcr_2018). Upregulation of all four genes was verified via qRT-PCR (Tcr_1019: 4-fold; Tcr_1315: ~7-fold; Tcr_0466: >200-fold; Tcr_2018: 7-fold), suggesting novel components are part of the response to nutrient limitation by this organism. In this study Thiomicrospira crunogena cells were grown under inorganic carbon limitation and ammonia limitation (high inorganic carbon concentrations) to examine genome wide transcription responses

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Dark accelerates dissolved inorganic phosphorus release of high-density cyanobacteria.

Publications

Dark accelerates dissolved inorganic phosphorus release of high-density cyanobacteria.

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