Ontology highlight
ABSTRACT:
SUBMITTER: Hardcastle T
PROVIDER: S-EPMC7755457 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Hardcastle Travis T Novosjolova Irina I Kotikam Venubabu V Cheruiyot Samwel K SK Mutisya Daniel D Kennedy Scott D SD Egli Martin M Kelley Melissa L ML Smith Anja van Brabant AVB Rozners Eriks E
ACS chemical biology 20180110 3
Potential in vivo applications of RNA interference (RNAi) require suppression of various off-target activities. Herein, we report that replacement of a single phosphate linkage between the first and second nucleosides of the passenger strand with an amide linkage almost completely abolished its undesired activity and restored the desired activity of guide strands that had been compromised by unfavorable amide modifications. Molecular modeling suggested that the observed effect was most likely du ...[more]