Ontology highlight
ABSTRACT:
SUBMITTER: Qing R
PROVIDER: S-EPMC7756140 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Qing Rui R Tao Fei F Chatterjee Pranam P Yang Gaojie G Han Qiuyi Q Chung Haeyoon H Ni Jun J Suter Bernhard P BP Kubicek Jan J Maertens Barbara B Schubert Thomas T Blackburn Camron C Zhang Shuguang S
iScience 20201028 12
It was posited that functionalities of GPCRs require full-length sequences that are negated by residue deletions. Here we report that significantly truncated nfCCR5<sup>QTY</sup> and nfCXCR4<sup>QTY</sup> still bind native ligands. Receptor-ligand interactions were discovered from yeast 2-hybrid screening and confirmed by mating selection. Two nfCCR5<sup>QTY</sup> (SZ218a, SZ190b) and two nfCXCR4<sup>QTY</sup> (SZ158a, SZ146a) were expressed in <i>E. coli</i>. Synthesized receptors exhibited α-h ...[more]