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Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells.

ABSTRACT: A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.

SUBMITTER: Robson JA

PROVIDER: S-EPMC7756414 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells.

Robson Jonathan A JA Kubánková Markéta M Bond Tamzin T Hendley Rian A RA White Andrew J P AJP Kuimova Marina K MK Wilton-Ely James D E T JDET

Angewandte Chemie (International ed. in English) 20200917 48

A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that c ...[more]

PMID: 32686308

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