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ABSTRACT:
SUBMITTER: Abbandonato G
PROVIDER: S-EPMC5961464 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Abbandonato Gerardo G Polli Dario D Viola Daniele D Cerullo Giulio G Storti Barbara B Cardarelli Francesco F Salomone Fabrizio F Nifosì Riccardo R Signore Giovanni G Bizzarri Ranieri R
Biophysical journal 20180501 9
Many intracellular reactions are dependent on the dielectric ("polarity") and viscosity properties of their milieu. Fluorescence imaging offers a convenient strategy to report on such environmental properties. Yet, concomitant and independent monitoring of polarity and viscosity in cells at submicron scale is currently hampered by the lack of fluorescence probes characterized by unmixed responses to both parameters. Here, the peculiar photophysics of a green fluorescent protein chromophore analo ...[more]