Ontology highlight
ABSTRACT:
SUBMITTER: Kitchen P
PROVIDER: S-EPMC7757292 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Kitchen Philip P Salman Mootaz M MM Abir-Awan Mohammed M Al-Jubair Tamim T Törnroth-Horsefield Susanna S Conner Alex C AC Bill Roslyn M RM
STAR protocols 20201102 3
Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenched in a concentration-dependent manner. Therefore, on short timescales, its concentration-dependent fluorescence can be used as a probe of cell volume, and therefore a probe of water transport into or ...[more]