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Genome-wide Interrogation of Protein-DNA Interactions in Mammalian Cells Using ChIPmentation.

ABSTRACT: Mapping the genomic locations of chromatin-associated proteins, such as transcription factors and histone modifications, is key to understanding the mechanisms of transcriptional regulation. ChIPmentation offers a simple and robust way of investigating the genomic binding sites of a protein using relatively low-input material. Here, we present a detailed protocol for the key steps that lead to a successful ChIPmentation experiment, as well as a quick analysis pipeline to examine the data. For complete details on the use and execution of this protocol, please refer to Schmidl et al. (2015). For example data produced by this protocol, please refer to Henriksson et al. (2019) and Zhang et al. (2019).

SUBMITTER: Xu W

PROVIDER: S-EPMC7757419 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Genome-wide Interrogation of Protein-DNA Interactions in Mammalian Cells Using ChIPmentation.

Xu Wei W Ye Ying Y Sharrocks Andrew D AD Zhang Wensheng W Chen Xi X

STAR protocols 20201204 3

Mapping the genomic locations of chromatin-associated proteins, such as transcription factors and histone modifications, is key to understanding the mechanisms of transcriptional regulation. ChIPmentation offers a simple and robust way of investigating the genomic binding sites of a protein using relatively low-input material. Here, we present a detailed protocol for the key steps that lead to a successful ChIPmentation experiment, as well as a quick analysis pipeline to examine the data. For co ...[more]

PMID: 33377081

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