Visualizing Cellular Dynamics and Protein Localization in 3D Collagen.
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ABSTRACT:
SUBMITTER: Koh WH
PROVIDER: S-EPMC7757667 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
Visualizing Cellular Dynamics and Protein Localization in 3D Collagen.
Koh Wan Hon WH Zayats Romaniya R Lopez Paul P Murooka Thomas T TT
STAR protocols 20201208 3
Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response. While studying these events in cell culture are standard procedure, spatiotemporal dynamics of cell-to-cell interactions within three-dimensional (3D) environments are critical in generating appropriate effector functions. Here, we present a detailed protocol to study cells within an all-in-one 3D collagen matrix that is amenable to live-cell micro ...[more]
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