A quantitative medium-throughput assay to measure Caenorhabditis elegans development and reproduction.
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ABSTRACT:
SUBMITTER: Wittkowski P
PROVIDER: S-EPMC7757729 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
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A quantitative medium-throughput assay to measure <i>Caenorhabditis elegans</i> development and reproduction.
Wittkowski Paul P Violet Norman N Oelgeschläger Michael M Schönfelder Gilbert G Vogl Silvia S
STAR protocols 20201216 3
Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in <i>Caenorhabditis elegans</i>. Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated framework using open-source software. The assay utilizes whole 96-well fluorescence images taken with a high-content screening system. Alternatively, conventional fluorescence images can also b ...[more]
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