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A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay.

ABSTRACT: The National Research Council has outlined the need for non-mammalian toxicological models to test the potential health effects of a large number of chemicals while also reducing the use of traditional animal models. The nematode Caenorhabditis elegans is an attractive alternative model because of its well-characterized and evolutionarily conserved biology, low cost, and ability to be used in high-throughput screening. A high-throughput method is described for quantifying the reproductive capacity of C. elegans exposed to chemicals for 48 h from the last larval stage (L4) to adulthood using a COPAS Biosort. Initially, the effects of exposure conditions that could influence reproduction were defined. Concentrations of DMSO vehicle

SUBMITTER: Boyd WA

PROVIDER: S-EPMC2871981 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay.

Boyd Windy A WA McBride Sandra J SJ Rice Julie R JR Snyder Daniel W DW Freedman Jonathan H JH

Toxicology and applied pharmacology 20100304 2

The National Research Council has outlined the need for non-mammalian toxicological models to test the potential health effects of a large number of chemicals while also reducing the use of traditional animal models. The nematode Caenorhabditis elegans is an attractive alternative model because of its well-characterized and evolutionarily conserved biology, low cost, and ability to be used in high-throughput screening. A high-throughput method is described for quantifying the reproductive capaci ...[more]

PMID: 20206647

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