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Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice.

ABSTRACT:

Background

The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes.

Methods

We used the CRISPR/Cas9 system to generate Dnajb8 mutant mice and analyze the phenotype of the Dnajb8 mutants.

Results

Although Dnajb8 is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups.

SUBMITTER: Wang F

PROVIDER: S-EPMC7759119 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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<i>Dnajb8</i>, a target gene of SOX30, is dispensable for male fertility in mice.

Wang Fengsong F Kong Shuai S Hu Xuechun X Li Xin X Xu Bo B Yue Qiuling Q Fu Kaiqiang K Ye Lan L Bai Shun S

PeerJ 20201221

<h4>Background</h4>The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of <i>Dnajb8</i> during late meiosis and spermiogenesis in mouse testes.<h4>Methods</h4>We used the CRISPR/Cas9 system to generate <i>Dnajb8</i> mutant mice and analyze the phenotype of the <i>Dnajb8</i> mutants.<h4>Results</h4>Although <i>Dnajb8</i> is an evolutionarily con ...[more]

PMID: 33391882

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