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The heat shock protein family gene Hspa1l in male mice is dispensable for fertility.

ABSTRACT: Background:Heat shock protein family A member 1 like (Hspa1l) is a member of the 70kD heat shock protein (Hsp70) family. HSPA1L is an ancient, evolutionarily conserved gene with a highly conserved domain structure. The gene is highly abundant and constitutively expressed in the mice testes. However, the role of Hspa1l in the testes has still not been elucidated. Methods:Hspa1l-mutant mice were generated using the CRISPR/Cas9 system. Histological and immunofluorescence staining were used to analyze the phenotypes of testis and epididymis. Apoptotic cells were detected through TUNEL assays. Fertility and sperm motilities were also tested. Quantitative RT-PCR was used for analyzing of candidate genes expression. Heat treatment was used to induce heat stress of the testis. Results:We successfully generated Hspa1l knockout mice. Hspa1l -/- mice exhibited normal development and fertility. Further, Hspa1l -/- mice shown no significant difference in spermatogenesis, the number of apoptotic cells in testes epididymal histology, sperm count and sperm motility from Hspa1l +/+ mice. Moreover, heat stress does not exacerbate the cell apoptosis in Hspa1l -/- testes. These results revealed that HSPA1L is not essential for physiological spermatogenesis, nor is it involved in heat-induced stress responses, which provides a basis for further studies.

SUBMITTER: Wang X

PROVIDER: S-EPMC7098389 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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The heat shock protein family gene <i>Hspa1l</i> in male mice is dispensable for fertility.

Wang Xin X Xie Wenxiu W Yao Yejin Y Zhu Yunfei Y Zhou Jianli J Cui Yiqiang Y Guo Xuejiang X Yuan Yan Y Zhou Zuomin Z Liu Mingxi M

PeerJ 20200323

<h4>Background</h4>Heat shock protein family A member 1 like (<i>Hspa1l</i>) is a member of the 70kD heat shock protein (<i>Hsp70</i>) family. HSPA1L is an ancient, evolutionarily conserved gene with a highly conserved domain structure. The gene is highly abundant and constitutively expressed in the mice testes. However, the role of <i>Hspa1l</i> in the testes has still not been elucidated.<h4>Methods</h4><i>Hspa1l</i>-mutant mice were generated using the CRISPR/Cas9 system. Histological and imm ...[more]

PMID: 32231871

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Project description:BackgroundIn living organisms, small heat shock proteins (sHSPs) are triggered in response to stress situations. This family of proteins is large in plants and, in the case of tomato (Solanum lycopersicum), 33 genes have been identified, most of them related to heat stress response and to the ripening process. Transcriptomic and proteomic studies have revealed complex patterns of expression for these genes. In this work, we investigate the coregulation of these genes by performing a computational analysis of their promoter architecture to find regulatory motifs known as heat shock elements (HSEs). We leverage the presence of sHSP members that originated from tandem duplication events and analyze the promoter architecture diversity of the whole sHSP family, focusing on the identification of HSEs.ResultsWe performed a search for conserved genomic sequences in the promoter regions of the sHSPs of tomato, plus several other proteins (mainly HSPs) that are functionally related to heat stress situations or to ripening. Several computational analyses were performed to build multiple sequence motifs and identify transcription factor binding sites (TFBS) homologous to HSF1AE and HSF21 in Arabidopsis. We also investigated the expression and interaction of these proteins under two heat stress situations in whole tomato plants and in protoplast cells, both in the presence and in the absence of heat shock transcription factor A2 (HsfA2). The results of these analyses indicate that different sHSPs are up-regulated depending on the activation or repression of HsfA2, a key regulator of HSPs. Further, the analysis of protein-protein interaction between the sHSP protein family and other heat shock response proteins (Hsp70, Hsp90 and MBF1c) suggests that several sHSPs are mediating alternative stress response through a regulatory subnetwork that is not dependent on HsfA2.ConclusionsOverall, this study identifies two regulatory motifs (HSF1AE and HSF21) associated with the sHSP family in tomato which are considered genomic HSEs. The study also suggests that, despite the apparent redundancy of these proteins, which has been linked to gene duplication, tomato sHSPs showed different up-regulation and different interaction patterns when analyzed under different stress situations.

Dataset Information

The heat shock protein family gene Hspa1l in male mice is dispensable for fertility.

Publications

The heat shock protein family gene <i>Hspa1l</i> in male mice is dispensable for fertility.

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