MRNA Expression and Role of PPAR? and PPAR? in Bovine Preimplantation Embryos Depending on the Quality and Developmental Stage.

ABSTRACT: Peroxisome proliferator-activated receptors (PPARs), a nuclear receptors for prostacyclin (PGI₂) have been recognized as being essential for early embryo development. The objectives of the present study were to determine if the bovine early- and late-cleaved embryos in different stages of early development express PPAR? and PPAR?. Since embryo developmental competence depends on numerous biological factors, we evaluated if the expression of PPAR? and PPAR? correlate with selected embryo quality markers (SOX2, OCT4, PLAC8, IGF1R) in the in vitro produced embryos at different stages of their development. Developmental rates and embryo quality for early- and late-cleaved embryos were provided according to International Embryo Transfer Society (IETS; developmental stages: 2-, 4-, 16-cell embryo, morula, blastocyst (1-early, 2-developing, 3-expanded, 4-hatched); quality stages: A-high quality, B-moderate quality, C-low quality). We found that bovine embryos expressed mRNA of PPAR? and PPAR? at all stages of early development, independently of their quality. In addition, the expression of PPAR? and PPAR? correlated with the expression of quality markers in bovine blastocysts. Positive correlations were stronger and more frequent in the group of early-cleaved embryos, whereas the negative correlations were typical for the group of late-cleaved embryos. Obtained results and available literature reports may indicate the participation of PGI₂, via PPAR? and PPAR?, in the processes related to the early embryo development, through the participation of this factor in the modulation of blastocyst hatching, implantation, and post-implantation development.

SUBMITTER: Suwik K 

PROVIDER: S-EPMC7763280 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature