Dataset Information

Greater Biofilm Formation and Increased Biodegradation of Polyethylene Film by a Microbial Consortium of Arthrobacter sp. and Streptomyces sp.

ABSTRACT: The widespread use of polyethylene (PE) mulch films has led to a significant accumulation of plastic waste in agricultural soils. The biodegradation of plastic waste by microorganisms promises to provide a cost-effective and environmentally-friendly alternative for mitigating soil plastic pollution. A large number of microorganisms capable of degrading PE have been reported, but degradation may be further enhanced by the cooperative activity of multiple microbial species. Here, two novel strains of Arthrobacter sp. and Streptomyces sp. were isolated from agricultural soils and shown to grow with PE film as a sole carbon source. Arthrobacter sp. mainly grew in the suspension phase of the culture, and Streptomyces sp. formed substantial biofilms on the surface of the PE film, indicating that these strains were of different metabolic types and occupied different microenvironments with contrasting nutritional access. Individual strains were able to degrade the PE film to some extent in a 90-day inoculation experiment, as indicated by decreased hydrophobicity, increased carbonyl index and CO₂ evolution, and the formation of biofilms on the film surface. However, a consortium of both strains had a much greater effect on these degradation properties. Together, these results provide new insights into the mechanisms of PE biodegradation by a microbial consortium composed of different types of microbes with possible metabolic complementarities.

SUBMITTER: Han YN

PROVIDER: S-EPMC7764375 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

Greater Biofilm Formation and Increased Biodegradation of Polyethylene Film by a Microbial Consortium of <i>Arthrobacter</i> sp. and <i>Streptomyces</i> sp.

Han Ya-Nan YN Wei Min M Han Fang F Fang Chao C Wang Dong D Zhong Yu-Jie YJ Guo Chao-Li CL Shi Xiao-Yan XY Xie Zhong-Kui ZK Li Feng-Min FM

Microorganisms 20201212 12

The widespread use of polyethylene (PE) mulch films has led to a significant accumulation of plastic waste in agricultural soils. The biodegradation of plastic waste by microorganisms promises to provide a cost-effective and environmentally-friendly alternative for mitigating soil plastic pollution. A large number of microorganisms capable of degrading PE have been reported, but degradation may be further enhanced by the cooperative activity of multiple microbial species. Here, two novel strains ...[more]

PMID: 33322790

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Project description:Petroleum oil is a complex mixture of substances, the majority of which are hydrocarbons; the latter represent an extremely important and heterogeneous group of compounds that find their way into water resources by anthropogenic or natural ways. The majority of toxic hydrocarbon components of petroleum are biodegradable, where bioremediation using microbial species has become an integral process for the restoration of oil-polluted areas. In this study, three bioremediation processes, namely natural attenuation, nutrient supplementation by adding glucose and biostimulation by adding Tween® 80, were carried out in various petroleum hydrocarbon concentrations in polluted water media using a consortium of three protozoan isolates (Aspidisca sp., Trachelophyllum sp. and Peranema sp.). A first-order kinetics model was fitted to the biodegradation data to evaluate the biodegradation rate and to determine the corresponding half-life time. First-order kinetics satisfactorily described the biodegradation of the petroleum-based contaminants under abiotic conditions. The results showed an increase in the percentage removal of petroleum oil at the lower petroleum concentrations and a gradual percentage decrease in removing petroleum oil residues occurred when there was an increase in the initial concentrations of the petroleum oil: 39%, 27%, 22%, 12%, 10% for various petroleum oil concentrations of 50, 100, 150, 200, 250 mg/L, respectively. A similar trend was also observed in the glucose-supplemented culture media where the reduction was 45% and 78% for petroleum concentrations of 250 mg/L and 50 mg/L, respectively. Biodegradation of between 33 and 90% was achieved at a Tween® 80 concentration of between 50 mg/L and 250 mg/L. The degradation rate constants for the natural attenuation process ranged between ≥0 to ≤0.50, ≥0 to ≤0.35, ≥0 to ≤0.25, ≥0 to ≤ 0.14 and ≥ 0 to ≤0.11 for petroleum oil concentrations varying from 50, 100, 150, 200 and 250 mg/L, respectively, during the study after 30 days. In the presence of glucose as a nutrient supplement, the degradation rate constants increased from 0 day-1 to 0.8 day-1 when exposed to the lowest oil concentration of 50 mg/L, while the lowest rate constants (from 0 day-1 to 0.25 day-1) were observed at the highest petroleum oil concentration of 250 mg/L for the same period. Overall enhancement of the degradation rates was achieved when adding the Tween® 80 surfactant compared to the first two biodegradation processes. The longest half-life was achieved after 217 days during the natural attenuation process for samples with an oil concentration of 250 mg/L and this was reduced to 85 days using the glucose-supplemented process. There was a further decrease to 45 days when Tween® 80 surfactant was added during the biostimulation process. The highest efficiency of ≥20% of Tween® 80 was observed between 6 and 18 days and thereafter it decreased slightly to ≤20%.

Dataset Information

Greater Biofilm Formation and Increased Biodegradation of Polyethylene Film by a Microbial Consortium of Arthrobacter sp. and Streptomyces sp.

Publications

Greater Biofilm Formation and Increased Biodegradation of Polyethylene Film by a Microbial Consortium of <i>Arthrobacter</i> sp. and <i>Streptomyces</i> sp.

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