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Discovery and Characterization of a Novel Ampelovirus on Firespike.


ABSTRACT: A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus Ampelovirus, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to Nicotiana benthamiana. In addition, a full-length cDNA clone of FLRaV could successfully infect N. benthamiana via agroinfiltration.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7766885 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Discovery and Characterization of a Novel Ampelovirus on Firespike.

Wang Yaqin Y   Song Yu Y   Wang Yongzhi Y   Cao Mengji M   Hu Tao T   Zhou Xueping X  

Viruses 20201216 12


A novel RNA virus was identified in firespike (<i>Odontonema tubaeforme</i>) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus <i>Ampelovirus</i>, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to <i>Nicotiana benthamiana</  ...[more]

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