Unknown

Dataset Information

0

Gamete binning: chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes.


ABSTRACT: Generating chromosome-level, haplotype-resolved assemblies of heterozygous genomes remains challenging. To address this, we developed gamete binning, a method based on single-cell sequencing of haploid gametes enabling separation of the whole-genome sequencing reads into haplotype-specific reads sets. After assembling the reads of each haplotype, the contigs are scaffolded to chromosome level using a genetic map derived from the gametes. We assemble the two genomes of a diploid apricot tree based on whole-genome sequencing of 445 individual pollen grains. The two haplotype assemblies (N50: 25.5 and 25.8?Mb) feature a haplotyping precision of greater than 99% and are accurately scaffolded to chromosome-level.

SUBMITTER: Campoy JA 

PROVIDER: S-EPMC7771071 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Gamete binning: chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes.

Campoy José A JA   Sun Hequan H   Goel Manish M   Jiao Wen-Biao WB   Folz-Donahue Kat K   Wang Nan N   Rubio Manuel M   Liu Chang C   Kukat Christian C   Ruiz David D   Huettel Bruno B   Schneeberger Korbinian K  

Genome biology 20201229 1


Generating chromosome-level, haplotype-resolved assemblies of heterozygous genomes remains challenging. To address this, we developed gamete binning, a method based on single-cell sequencing of haploid gametes enabling separation of the whole-genome sequencing reads into haplotype-specific reads sets. After assembling the reads of each haplotype, the contigs are scaffolded to chromosome level using a genetic map derived from the gametes. We assemble the two genomes of a diploid apricot tree base  ...[more]

Similar Datasets

| S-EPMC6476705 | biostudies-literature
| S-EPMC7954703 | biostudies-literature
| S-EPMC10687446 | biostudies-literature
| S-EPMC9464699 | biostudies-literature
| S-EPMC9447482 | biostudies-literature
| S-EPMC9834249 | biostudies-literature
| PRJEB37669 | ENA
| S-EPMC10724166 | biostudies-literature
| S-EPMC10784532 | biostudies-literature
| S-EPMC9135360 | biostudies-literature