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Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus.

ABSTRACT: We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention's Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.

SUBMITTER: Velusamy S

PROVIDER: S-EPMC7771451 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Changes in Group A Streptococcus emm Types Associated with Invasive Infections in Adults, Spain, 2023.

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Longitudinal Analysis of Group A Streptococcus emm Types and emm Clusters in a High-Prevalence Setting: Relationship between Past and Future Infections.

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High Incidence of Invasive Group A Streptococcus Disease Caused by Strains of Uncommon emm Types in Thunder Bay, Ontario, Canada.

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Identification of a chimeric emm gene and novel emm pattern in currently circulating strains of emm4 Group A Streptococcus.

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