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Detection of meat from horse, donkey and their hybrids (mule/hinny) by duplex real-time fluorescent PCR.

ABSTRACT: Meat adulteration is currently a common practice worldwide. In China, adulteration of donkey meat products with the similar species (horse and mule/hinny) meat and mislabeling are becoming widespread concerns. In this study, a sensitive and species-specific duplex real-time PCR assay based on the simultaneous amplification of fragments of the creatine kinase muscle gene family, was developed and optimized for the identification of horse, donkey and mule /hinny species in raw and heat-processed meat products. Duplex real-time PCR results showed different fluorescence amplification curves for horse and donkey. Both kinds of fluorescence amplification curves appeared simultaneously for mule/hinny. The limit of detection (LOD) of the method was up to 0.01 ng /?L. The method and strategy developed in this study could be applied to detect the presence of adulterants from horse and mule /hinny meat in raw donkey meat and meat products.

SUBMITTER: Wang D

PROVIDER: S-EPMC7771862 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Detection of meat from horse, donkey and their hybrids (mule/hinny) by duplex real-time fluorescent PCR.

Wang Dan D Wang Liping L Xue Chenyu C Han Yuebei Y Li Hejing H Geng Jianqiang J Jie Jiang J

PloS one 20201229 12

Meat adulteration is currently a common practice worldwide. In China, adulteration of donkey meat products with the similar species (horse and mule/hinny) meat and mislabeling are becoming widespread concerns. In this study, a sensitive and species-specific duplex real-time PCR assay based on the simultaneous amplification of fragments of the creatine kinase muscle gene family, was developed and optimized for the identification of horse, donkey and mule /hinny species in raw and heat-processed m ...[more]

PMID: 33373374

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