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EPySeg: a coding-free solution for automated segmentation of epithelia using deep learning.


ABSTRACT: Epithelia are dynamic tissues that self-remodel during their development. During morphogenesis, the tissue-scale organization of epithelia is obtained through a sum of individual contributions of the cells constituting the tissue. Therefore, understanding any morphogenetic event first requires a thorough segmentation of its constituent cells. This task, however, usually involves extensive manual correction, even with semi-automated tools. Here, we present EPySeg, an open-source, coding-free software that uses deep learning to segment membrane-stained epithelial tissues automatically and very efficiently. EPySeg, which comes with a straightforward graphical user interface, can be used as a Python package on a local computer, or on the cloud via Google Colab for users not equipped with deep-learning compatible hardware. By substantially reducing human input in image segmentation, EPySeg accelerates and improves the characterization of epithelial tissues for all developmental biologists.

SUBMITTER: Aigouy B 

PROVIDER: S-EPMC7774881 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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EPySeg: a coding-free solution for automated segmentation of epithelia using deep learning.

Aigouy Benoit B   Cortes Claudio C   Liu Shanda S   Prud'Homme Benjamin B  

Development (Cambridge, England) 20201223 24


Epithelia are dynamic tissues that self-remodel during their development. During morphogenesis, the tissue-scale organization of epithelia is obtained through a sum of individual contributions of the cells constituting the tissue. Therefore, understanding any morphogenetic event first requires a thorough segmentation of its constituent cells. This task, however, usually involves extensive manual correction, even with semi-automated tools. Here, we present EPySeg, an open-source, coding-free soft  ...[more]

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