Unknown

Dataset Information

0

Photosensitizer-based multimodal PSMA-targeting ligands for intraoperative detection of prostate cancer.


ABSTRACT: Incomplete resection of prostate cancer (PCa) occurs in 15%-50% of PCa patients. Disease recurrence negatively impacts oncological outcome. The use of radio-, fluorescent-, or photosensitizer-labeled ligands to target the prostate-specific membrane antigen (PSMA) has become a well-established method for the detection and treatment of PCa. Methods: Here, we developed and characterized multimodal [111In]In-DOTA(GA)-IRDye700DX-PSMA ligands, varying in their molecular composition, for use in intraoperative radiodetection, fluorescence imaging and targeted photodynamic therapy of PCa lesions. PSMA-specificity of these ligands was determined in xenograft tumor models and on fresh human PCa biopsies. Results: Ligand structure optimization showed that addition of the photosensitizer (IRDye700DX) and additional negative charges significantly increased ligand uptake in PSMA-expressing tumors. Moreover, an ex vivo incubation study on human tumor biopsies confirmed the PSMA-specificity of these ligands on human samples, bridging the gap to the clinical situation. Conclusion: We developed a novel PSMA-targeting ligand, optimized for multimodal image-guided PCa surgery combined with targeted photodynamic therapy.

SUBMITTER: Derks YHW 

PROVIDER: S-EPMC7778589 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

altmetric image

Publications


Incomplete resection of prostate cancer (PCa) occurs in 15%-50% of PCa patients. Disease recurrence negatively impacts oncological outcome. The use of radio-, fluorescent-, or photosensitizer-labeled ligands to target the prostate-specific membrane antigen (PSMA) has become a well-established method for the detection and treatment of PCa. <b>Methods:</b> Here, we developed and characterized multimodal [<sup>111</sup>In]In-DOTA(GA)-IRDye700DX-PSMA ligands, varying in their molecular composition,  ...[more]

Similar Datasets

| S-EPMC8778659 | biostudies-literature
| S-EPMC7060305 | biostudies-literature
| S-EPMC5728657 | biostudies-literature
| S-EPMC4659400 | biostudies-literature
| S-EPMC3534887 | biostudies-literature
| S-EPMC5581714 | biostudies-literature
| S-EPMC10895373 | biostudies-literature
| S-EPMC3526141 | biostudies-literature
| S-EPMC8355555 | biostudies-literature
| S-EPMC8490425 | biostudies-literature