Dataset Information

Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?

ABSTRACT: The volumetric oxygen mass transfer coefficient ([Formula: see text]) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched [Formula: see text]. The maximum [Formula: see text] of the BioFlo® 320 reusable bioreactor was 109 h^-1, which was approximately twice that of the BioBLU 0.3f single-use vessel. The results here show no overlap in [Formula: see text] values when both bioreactors were compared and thus conclude that scalability based on [Formula: see text] is not viable. The maximum [Formula: see text] of the Eppendorf BioBLU 0.3f single-use reported here was 47 h^-1 compared to that of the manufacturer's value of 2500 h^-1, indicating a 53-fold difference. This discrepancy was attributed to the incompatible sulfite addition method used by the manufacturer for estimation.

SUBMITTER: Olughu W

PROVIDER: S-EPMC7779418 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?

Olughu Williams W Galbraith Duncan D Paget Cillian C Ruscoe Steve S Smith Josh J Mason Alison A

World journal of microbiology & biotechnology 20210104 1

The volumetric oxygen mass transfer coefficient ([Formula: see text]) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched [Formula: see text]. The maximum [Formula: see text] of the BioFlo® 320 reusable bioreactor was 109 ...[more]

PMID: 33392800

Similar Datasets

Project description:BackgroundEthanol production through fermentation of gas mixtures containing CO, CO2 and H2 has just started operating at commercial scale. However, quantitative schemes for understanding and predicting productivities, yields, mass transfer rates, gas flow profiles and detailed energy requirements have been lacking in literature; such are invaluable tools for process improvements and better systems design. The present study describes the construction of a hybrid model for simulating ethanol production inside a 700 m3 bubble column bioreactor fed with gas of two possible compositions, i.e., pure CO and a 3:1 mixture of H2 and CO2.ResultsEstimations made using the thermodynamics-based black-box model of microbial reactions on substrate threshold concentrations, biomass yields, as well as CO and H2 maximum specific uptake rates agreed reasonably well with data and observations reported in literature. According to the bioreactor simulation, there is a strong dependency of process performance on mass transfer rates. When mass transfer coefficients were estimated using a model developed from oxygen transfer to water, ethanol productivity reached 5.1 g L-1 h-1; when the H2/CO2 mixture is fed to the bioreactor, productivity of CO fermentation was 19% lower. Gas utilization reached 23 and 17% for H2/CO2 and CO fermentations, respectively. If mass transfer coefficients were 100% higher than those estimated, ethanol productivity and gas utilization may reach 9.4 g L-1 h-1 and 38% when feeding the H2/CO2 mixture at the same process conditions. The largest energetic requirements for a complete manufacturing plant were identified for gas compression and ethanol distillation, being higher for CO fermentation due to the production of CO2.ConclusionsThe thermodynamics-based black-box model of microbial reactions may be used to quantitatively assess and consolidate the diversity of reported data on CO, CO2 and H2 threshold concentrations, biomass yields, maximum substrate uptake rates, and half-saturation constants for CO and H2 for syngas fermentations by acetogenic bacteria. The maximization of ethanol productivity in the bioreactor may come with a cost: low gas utilization. Exploiting the model flexibility, multi-objective optimizations of bioreactor performance might reveal how process conditions and configurations could be adjusted to guide further process development.

Dataset Information

Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?

Publications

Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets