Does the BioBLU 0.3f single-use scale to the BioFlo® 320 reuseable bioreactor on a matched volumetric oxygen mass transfer coefficient?

ABSTRACT: The volumetric oxygen mass transfer coefficient ([Formula: see text]) is an essential parameter in aerobic high-cell density fermentation where the availability of oxygen to growing microorganisms is a limiting factor. Bioprocess teams looking to scale-up/down between the Eppendorf BioBLU 0.3f single-use vessel and the BioFlo® 320 reusable vessel bioreactors may find it challenging using a matched [Formula: see text]. The maximum [Formula: see text] of the BioFlo® 320 reusable bioreactor was 109 h^-1, which was approximately twice that of the BioBLU 0.3f single-use vessel. The results here show no overlap in [Formula: see text] values when both bioreactors were compared and thus conclude that scalability based on [Formula: see text] is not viable. The maximum [Formula: see text] of the Eppendorf BioBLU 0.3f single-use reported here was 47 h^-1 compared to that of the manufacturer's value of 2500 h^-1, indicating a 53-fold difference. This discrepancy was attributed to the incompatible sulfite addition method used by the manufacturer for estimation.

SUBMITTER: Olughu W 

PROVIDER: S-EPMC7779418 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature