Ontology highlight
ABSTRACT:
SUBMITTER: Kingston NM
PROVIDER: S-EPMC7781096 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Kingston Nathan M NM Tilston-Lunel Andrew M AM Hicks-Berthet Julia J Varelas Xaralabos X
Methods in molecular biology (Clifton, N.J.) 20190101
The transcriptional coactivator with PDZ-binding motif (TAZ), which is encoded by the WWTR1 gene, is a key transcriptional effector of the Hippo signaling pathway. TAZ function has been implicated in a variety of developmental processes and diseases, most notably in driving oncogenesis. Given that nuclear-cytoplasmic localization dynamics dictate TAZ activity, techniques for visualizing TAZ localization are critical for its study. Here we describe an immunofluorescence microscopy protocol that a ...[more]