Functional Water Wires Catalyze Long-Range Proton Pumping in the Mammalian Respiratory Complex I.

ABSTRACT: The respiratory complex I is a gigantic (1 MDa) redox-driven proton pump that reduces the ubiquinone pool and generates proton motive force to power ATP synthesis in mitochondria. Despite resolved molecular structures and biochemical characterization of the enzyme from multiple organisms, its long-range (∼300 Å) proton-coupled electron transfer (PCET) mechanism remains unsolved. We employ here microsecond molecular dynamics simulations to probe the dynamics of the mammalian complex I in combination with hybrid quantum/classical (QM/MM) free energy calculations to explore how proton pumping reactions are triggered within its 200 Å wide membrane domain. Our simulations predict extensive hydration dynamics of the antiporter-like subunits in complex I that enable lateral proton transfer reacti

SUBMITTER: Ropke M 

PROVIDER: S-EPMC7785131 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature