Unknown

Dataset Information

0

Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells.


ABSTRACT:

Background

Anti-apoptotic protein Bcl-2 plays a substantial role in the carcinogenesis, whereas the regulation for Bcl-2 in gastric carcinoma (GC) is poorly understood. Specifically, a role of microRNA (miR)-383 in the control of Bcl-2 has not been shown in GC and thus addressed in the current study.

Methods

We investigated the levels of miR-383 and Bcl-2 in 50 GC specimens, and compared them with patients' clinical characteristics. Bioinformatics analyses and luciferase-reporter assay were applied for analyzing the relationship between Bcl-2 and miR-383. An CCK assay was used to determine the survival of Fluorouracil-treated GC cells, and apoptosis of GC cells was assessed by flow cytometric FITC Annexin V apoptosis detection assay and expression of apoptosis-associated proteins.

Results

The levels of miR-383 were lower while the levels of Bcl-2 levels were higher in GC specimens, compared to tissue from the adjacent non-tumor region. Low miR-383 and high Bcl-2 seemed to be associated with high malignancy and metastasis. In GC specimens, the levels of Bcl-2 and miR-383 inversely correlated. The overall survival of miR-383-low cases was poorer. Mechanistically, miR-383 targeted the 3'-UTR of Bcl-2 mRNA to inhibit its protein translation. Overexpression of miR-383 downregulated Bcl-2, resulting in reduced survival of Fluorouracil-treated GC cells. Similar conclusion was drawn through analysis of published database.

Conclusion

MiR-383 reduces survival of Fluorouracil-treated GC cells through downregulating of Bcl-2.

SUBMITTER: Tao S 

PROVIDER: S-EPMC7786514 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Translational control of Bcl-2 promotes apoptosis of gastric carcinoma cells.

Tao Shuangfen S   Gu Jianchun J   Wang Qing Q   Zheng Leizhen L  

BMC cancer 20210105 1


<h4>Background</h4>Anti-apoptotic protein Bcl-2 plays a substantial role in the carcinogenesis, whereas the regulation for Bcl-2 in gastric carcinoma (GC) is poorly understood. Specifically, a role of microRNA (miR)-383 in the control of Bcl-2 has not been shown in GC and thus addressed in the current study.<h4>Methods</h4>We investigated the levels of miR-383 and Bcl-2 in 50 GC specimens, and compared them with patients' clinical characteristics. Bioinformatics analyses and luciferase-reporter  ...[more]

Similar Datasets

| S-EPMC6990914 | biostudies-literature
| S-EPMC2739939 | biostudies-literature
| S-EPMC5667555 | biostudies-literature
| S-EPMC7059858 | biostudies-literature
| S-EPMC9861585 | biostudies-literature
| S-EPMC7293391 | biostudies-literature
| S-EPMC8521017 | biostudies-literature
| S-EPMC8072958 | biostudies-literature
| S-EPMC3953594 | biostudies-literature
| S-EPMC4074544 | biostudies-literature